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immpress hrp polymer anti mouse igg reagent  (Vector Laboratories)


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    Structured Review

    Vector Laboratories immpress hrp polymer anti mouse igg reagent
    Immpress Hrp Polymer Anti Mouse Igg Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/MPX-2402-15/Mouse+on+Mouse+(M%2EO%2EM%2E+)+ImmPRESS+Peroxidase+Polymer+Anti-Mouse+Reagent/pm41385584-352-10-16
    Average 93 stars, based on 149 article reviews
    immpress hrp polymer anti mouse igg reagent - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Folliculin deletion in the mouse kidney results in cystogenesis of the loops of Henle via aberrant TFEB activation.
    Article Snippet: The nuclei were counterstained with 4,6-diamidino-2phenylindole (DAPI) (Dojindo, Kumamoto, Japan, #NQ215). .. The following primary antibodies were used: rabbit anti-SIX2 (Proteintech, Rosemont, Illinois, #11562-1-AP; 1:400 dilution ( , rat anti-KRT8 (DSHB, Iowa, United States; 1:10), mouse anti-E-cadherin (CDH1) (BD, Franklin Lakes, New Jersey, #610181; 1:100); rabbit anti-NKCC2 (SLC12A1) (StressMarq Bioscience, Victoria, Canada, #SPC-401D; 1:100), goat anti-NKCC2 (SLC12A1) (Invitrogen, Carlesbad, California, #PAS-142445; 1:100), rabbit anti-phospho S6 ribosomal protein (Ser Jo urn al Pr -pr oo f 10 240/244) (Cell Signaling, Danvers, Massachusetts, #5364 ; 1:100), rabbit anti-WT1 (Abcam, Cambridge, UK, #GR 3270281-4; 1:100), LTL (VECTOR, Newark, California, #B-132; 1:200), guinea pig anti-NEPHRIN (Progen, Heidelberg, Germany, #GP-N2, 1:200), rabbit anti-TFE3 (Sigma-Aldrich, St. Louis, Minnesota, #HPA023881; 1:100), and rabbit anti-TFEB antibodies (Bethyl Laboratories, Montgomery, Texas, #A303-673A, Lot No. 8 and 9).10 For staining of TFE3 and TFEB, sections were incubated with the primary antibodies followed by an antirabbit secondary antibody conjugated with peroxidase polymers from an ImmPress Reagent Kit (VECTOR Laboratories; #MP-7401). .. The sections were then incubated with Alexa Fluor 594- tyramide (Thermo Fisher Scientific, Waltham, Massachusetts, #B40957) for 15 min at room temperature.

    Incubation:

    Article Title: Folliculin deletion in the mouse kidney results in cystogenesis of the loops of Henle via aberrant TFEB activation.
    Article Snippet: The nuclei were counterstained with 4,6-diamidino-2phenylindole (DAPI) (Dojindo, Kumamoto, Japan, #NQ215). .. The following primary antibodies were used: rabbit anti-SIX2 (Proteintech, Rosemont, Illinois, #11562-1-AP; 1:400 dilution ( , rat anti-KRT8 (DSHB, Iowa, United States; 1:10), mouse anti-E-cadherin (CDH1) (BD, Franklin Lakes, New Jersey, #610181; 1:100); rabbit anti-NKCC2 (SLC12A1) (StressMarq Bioscience, Victoria, Canada, #SPC-401D; 1:100), goat anti-NKCC2 (SLC12A1) (Invitrogen, Carlesbad, California, #PAS-142445; 1:100), rabbit anti-phospho S6 ribosomal protein (Ser Jo urn al Pr -pr oo f 10 240/244) (Cell Signaling, Danvers, Massachusetts, #5364 ; 1:100), rabbit anti-WT1 (Abcam, Cambridge, UK, #GR 3270281-4; 1:100), LTL (VECTOR, Newark, California, #B-132; 1:200), guinea pig anti-NEPHRIN (Progen, Heidelberg, Germany, #GP-N2, 1:200), rabbit anti-TFE3 (Sigma-Aldrich, St. Louis, Minnesota, #HPA023881; 1:100), and rabbit anti-TFEB antibodies (Bethyl Laboratories, Montgomery, Texas, #A303-673A, Lot No. 8 and 9).10 For staining of TFE3 and TFEB, sections were incubated with the primary antibodies followed by an antirabbit secondary antibody conjugated with peroxidase polymers from an ImmPress Reagent Kit (VECTOR Laboratories; #MP-7401). .. The sections were then incubated with Alexa Fluor 594- tyramide (Thermo Fisher Scientific, Waltham, Massachusetts, #B40957) for 15 min at room temperature.

    Article Title: Derepression of the epithelial transcription factor GRHL2 promotes direct hepatocyte-to-cholangiocyte transdifferentiation
    Article Snippet: .. Subsequently, tissue sections were incubated 30 min at RT with ImmPRESS HRP Polymer anti-mouse IgG Reagent (Vector Laboratories), followed by a TBS wash. Signal was revealed with diaminobenzidine [DAB (Cell Signaling)], and sections were counterstained with hematoxylin (solution Gill N°1, Sigma-Aldrich) and mounted in Aquatex (Sigma-Aldrich). .. Stained slices were imaged with an Axioscan Z1 slide scanner (Zeiss).

    Polymer:

    Article Title: Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats
    Article Snippet: After incubation in 2.5% normal horse serum for 20 min, primary antibodies properly diluted in Animal-Free Blocker solution (Vector Laboratories) were applied at 4°C overnight ( ). .. The next day, after washing slides, secondary antibody ImmPRESS Polymer Reagent (either anti-rabbit or anti-mouse) was added for 30 min. After several PBS baths, revelation was carried by Vector DAB (3,3’-Diaminobenzidine) substrate. ..

    Article Title: Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats.
    Article Snippet: After incubation in 2.5% normal horse serum for 20 min, primary antibodies properly diluted in Animal-Free Blocker solution (Vector Laboratories) were applied at 4°C overnight (Table 2). .. The next day, after washing slides, secondary antibody ImmPRESS Polymer Reagent (either anti-rabbit or anti-mouse) was added for 30 min. After several PBS baths, revelation was carried by Vector DAB (3,3’-Diaminobenzidine) substrate. ..

    Article Title: Derepression of the epithelial transcription factor GRHL2 promotes direct hepatocyte-to-cholangiocyte transdifferentiation
    Article Snippet: .. Subsequently, tissue sections were incubated 30 min at RT with ImmPRESS HRP Polymer anti-mouse IgG Reagent (Vector Laboratories), followed by a TBS wash. Signal was revealed with diaminobenzidine [DAB (Cell Signaling)], and sections were counterstained with hematoxylin (solution Gill N°1, Sigma-Aldrich) and mounted in Aquatex (Sigma-Aldrich). .. Stained slices were imaged with an Axioscan Z1 slide scanner (Zeiss).

    Article Title: Expression, prognosis and preliminary investigation of the mechanism of action of ACTR6, a member of the ARPs gene family, in hepatocellular carcinoma
    Article Snippet: The rabbit polyclonal antibody against ACTR6 (Catalog # PA5-58453) was from Thermo Fisher Scientific (Waltham, MA). .. ImmPRESS Universal Polymer Reagent (Horse Anti-Mouse/Rabbit IgG) was from Vector Laboratories (S.F, CA). .. SiRNAs were transfected using the lipofectamine 3000 Transfection Kit (ThermoFisher Scientific, #L3000-015) after being obtained from Hippobio in Huzhou, China.

    Article Title: Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiency
    Article Snippet: .. For detection of the twin-Strep tag on constructs, the StrepTagII C23.21 antibody was visualized using the Mouse on Mouse ImmPress HRP Polymer kit (Vector Laboratories) according to the manufacturer’s protocol. .. Tissue slides were imaged with a slide scanner (Leica Aperio AT2) and the IHC signal of cortical, thalamic, and hippocampal regions were cropped from a whole coronal section image.

    other:

    Article Title: ARHGAP12 and ARHGAP29 exert distinct regulatory effects on switching between two cell morphological states through GSK-3 activity.
    Article Snippet: The chromogen substrate used was 3, 3’diaminobenzidine (ImmPACT DAB Peroxidase Substrate Kit by VECTOR laboratories, Orton Southgate, UK; Cat # SK-4100).

    Article Title: Derepression of the epithelial transcription factor GRHL2 promotes direct hepatocyte-to-cholangiocyte transdifferentiation.
    Article Snippet: Protein A/G sepharose beads (GE Healthcare) were added and samples were incubated for 4 h at 4°C under agitation in the presence of 10 μg/mL yeast tRNA (Sigma-Aldrich, R5636).

    Construct:

    Article Title: Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiency
    Article Snippet: .. For detection of the twin-Strep tag on constructs, the StrepTagII C23.21 antibody was visualized using the Mouse on Mouse ImmPress HRP Polymer kit (Vector Laboratories) according to the manufacturer’s protocol. .. Tissue slides were imaged with a slide scanner (Leica Aperio AT2) and the IHC signal of cortical, thalamic, and hippocampal regions were cropped from a whole coronal section image.



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